Single-Cell Cut&Tag Reagent Kits

Single-Cell Cut&Tag Reagent Kits empower researchers to perform precise epigenetic profiling at the single-cell level, leveraging cutting-edge technologies without the necessity for additional platforms or third-party kits. These kits facilitate cost-effective and high-throughput analysis of chromatin modifications and other DNA-associated proteins, seamlessly integrating with leading single-cell sequencing systems.

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Why Choose Single-Cell Cut&Tag from Epigenome Technologies?

• Platform Flexibility: Compatible with leading single-cell sequencing platforms, providing versatility across different laboratory settings.

• High Quality and Reproducibility: Rigorous quality control ensures consistent and reliable data generation.

• Cost-Effective Solutions: Eliminate the need for additional platforms or kits, reducing overall research costs.

• Comprehensive Reagent Kits: All-in-one kits include everything needed for end-to-end assay execution, from sample preparation to library sequencing.

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Droplet scCut&Tag

Droplet scCut&Tag is optimized for high-resolution epigenetic profiling using droplet-based single-cell platforms. This kit is fully compatible with the 10X ATAC Kit and the Chromium System, ensuring streamlined workflows and reliable data quality.

• Key Features:

  • Compatibility: Designed to integrate effortlessly with the 10X Genomics ATAC Kit and Chromium Single Cell System.

  • High Throughput: Enables the processing of thousands of single cells simultaneously, maximizing data output and efficiency.

  • Comprehensive Profiling: Simultaneously maps chromatin accessibility and specific histone modifications, providing a multifaceted view of the epigenome.

  • User-Friendly Protocol: Detailed protocols and reagents simplify the workflow, reducing hands-on time and potential errors.

• Workflow Overview:

  1. Cell Preparation: Isolate single cells and prepare them for the Cut&Tag assay.

  2. Antibody Targeting: Utilize specific antibodies conjugated to pAG-Tn5 transposase to bind desired histone modifications.

  3. Tagmentation: Perform tagmentation within droplets, integrating seamlessly with the 10X workflow.

  4. Library Preparation: Generate sequencing-ready libraries compatible with Chromium sequencing platforms.

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Emulsion scCut&Tag

Emulsion scCut&Tag is tailored for robust epigenetic analyses using emulsion-based single-cell platforms. This kit is specifically compatible with the Fluent PiPseq single-cell platform, offering flexibility and precision for diverse research applications.

• Key Features:

  • Platform Compatibility: Engineered to work seamlessly with the Fluent PiPseq single-cell platform, ensuring optimal performance and data integration.

  • Versatile Applications: Suitable for a wide range of tissues and cell types, facilitating diverse epigenetic studies.

  • Scalable Solutions: Supports both small-scale experiments and large-scale studies, accommodating varying research needs.

  • Comprehensive Reagents: Includes all necessary components for efficient assay execution, from cell lysis to library construction.

• Workflow Overview:

  1. Cell Encapsulation: Encapsulate single cells within emulsion droplets using the Fluent PiPseq system.

  2. Antibody Binding: Apply antibodies linked to pAG-Tn5 transposase to target specific histone modifications within each droplet.

  3. In-Droplet Tagmentation: Conduct tagmentation within the emulsion, ensuring precise integration of sequencing adapters.

  4. Library Construction: Proceed to library amplification and purification, yielding high-quality, sequencing-ready libraries.

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Comprehensive Support and Services

At Epigenome Technologies, we are dedicated to supporting your research endeavors with our Single-Cell Cut&Tag products. Our team collaborates closely with investigators to provide:

• Detailed Protocols: Step-by-step instructions tailored to optimize assay performance for various tissues, targets, and antibodies.

• Technical Support: Expert assistance to troubleshoot and refine your experimental workflows.

• Custom Services: For researchers preferring to outsource their assays, we offer comprehensive Single-Cell Cut&Tag services. Our expertise ensures precise and reliable results, allowing you to focus on data analysis and interpretation without the operational complexities of running the assays in-house.